試管嬰兒的科技 - 微型注射法

單精子卵漿內注射(Intracytoplasmic sperm injection)

此療程包括刺激卵巢以產生多個卵子，以便在超聲波引導下抽取出來。
進行單精子卵漿內注

­射是挑選單一條精子繼而注射入卵子內以逹至受精。

結合的受精卵子經過2-3天的發展，然後選擇形成的胚胎移植到子宮。
移植後，剩餘適合­的胚胎會冷藏作未來備用。




單精子卵漿內注射ICSI 可應用於以下情況:

• 精子數目極少
• 精子活動能力低
• 極少正常精子
• 曾經嘗試體外受精(IVF)但卵子未能受精
• 需要經過手術直接從睾丸或副睾取得精子

單精子卵漿內注射包括以下程序:

• 核對婚姻狀況
• 評估及輔導
• 檢查包括血型、全血檢查、德國麻疹抗體、乙型肝炎抗原及丙型肝炎抗體、
  愛滋病抗原/抗­體及梅毒(VDRL)檢驗
• 荷爾蒙檢查
• 簽署同意書
• 利用激素促使卵巢產生多個卵子
• 使用利用超聲波監察卵泡發展，及有需要時檢查血清雌二醇(oestradiol)水平
• 在超聲波監察下進行取卵
• 單精子卵漿內注射需要篩選個別活躍的精子，然後注射每個成熟的卵子內
• 移植胚胎到子宮內
• 黃體期支持以助子宮內膜增長及胚胎著床

參考資料:www.reprodmed.com
以上所提供的資訊僅作為教育及參考用途，如果你有任何醫療問題，
應向自己的婦產科醫生查詢，而不應單倚賴以上提供的資料。

儲存器皿 - 確保取出的卵子溫差縮小

冷藏卵子, 卵子冷藏...為了保存女性的生育能力，冷藏卵子是其中一個方法。

近年有很多硏究報告­指出玻璃化冷凍(Vitrification)卵子技術的成功率十分高，需要時可以將­這些玻璃化冷凍的卵子融解，再以細胞漿内精子注入法(ICSI)把精子注入卵子内，培­養後的胚胎可以植入子宮而令女性懷孕。

這些經過玻璃化冷凍的卵子成功的懷孕率與採用新­鮮的卵子相若。而根據The Practice Committees of the American Society for Reproductive Medicine的建議，這技術已完成實驗階段，可以應用到有需要的女士身上。





參考資料:www.reprodmed.com
以上所提供的資訊僅作為教育及參考用途，如果你有任何醫療問題，
應向自己的婦產科醫生查詢，而不應單倚賴以上提供的資料。

試管嬰兒的科技 - 微型注射法

單精子卵漿內注射(Intracytoplasmic sperm injection)

此療程包括刺激卵巢以產生多個卵子，以便在超聲波引導下抽取出來。
進行單精子卵漿內注­射是挑選單一條精子繼而注射入卵子內以逹至受精。

結合的受精卵子經過2-3天的發展，然後選擇形成的胚胎移植到子宮。
移植後，剩餘適合­的胚胎會冷藏作未來備用。




單精子卵漿內注射ICSI 可應用於以下情況:

• 精子數目極少
• 精子活動能力低
• 極少正常精子
• 曾經嘗試體外受精(IVF)但卵子未能受精
• 需要經過手術直接從睾丸或副睾取得精子

單精子卵漿內注射包括以下程序:

• 核對婚姻狀況
• 評估及輔導
• 檢查包括血型、全血檢查、德國麻疹抗體、乙型肝炎抗原及丙型肝炎抗體、
  愛滋病抗原/抗­體及梅毒(VDRL)檢驗
• 荷爾蒙檢查
• 簽署同意書
• 利用激素促使卵巢產生多個卵子
• 使用利用超聲波監察卵泡發展，及有需要時檢查血清雌二醇(oestradiol)水平
• 在超聲波監察下進行取卵
• 單精子卵漿內注射需要篩選個別活躍的精子，然後注射每個成熟的卵子內
• 移植胚胎到子宮內
• 黃體期支持以助子宮內膜增長及胚胎著床

參考資料:www.reprodmed.com
以上所提供的資訊僅作為教育及參考用途，如果你有任何醫療問題，
應向自己的婦產科醫生查詢，而不應單倚賴以上提供的資料。

(Video) Infertility treatment - IVF/ ICSI

It involves ovarian stimulation to produce a number of eggs, which are collected under ultrasound guidance. With IVF, the eggs are mixed with prepared sperm and fertilization takes place over the next few hours. With lCSl, a single selected sperm is injected into the egg to fertilize it. The resulting fertilised eggs are allowed to develop for 23 days Embryos are selected for transfer in to the uterus. Following embryo transfer, any remaining suitable embryos are cryopreserved for future transfer.

Indications for IVF may include Blocked, damaged or absent fallopian tubes; Endometriosis; Ovulatory problem; Unexplained infertility; and Sperm problems. .








Reference: fertility.com.hk

The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.

The IVF process / ICSI procedure consists of the following stages

The IVF process / ICSI procedure consists of the following stages:

 Confirmation of marital status;
 Assessment and counseling;
 Investigations including blood group, full blood count, Rubella antibody, hepatitis B and C antibodies, HIV antigen/antibody and VDRL;
 Hormone assays;
 Signing of c onsent forms;
 Ovarian stimulation using hormone stimulating hormone to produce a number of eggs;
 Monitoring ovarian response by ultrasound and serum oestradiol level as indicated;
 Collection of eggs under ultrasound guidance;
 Preparation of the sperm for combining with the eggs in the laboratory, leading to fertilization. For ICSI, individual sperm are isolated and injected into each mature egg;
 Replacement of embryos into the uterus.
 Luteal support to assist endometrial preparation and subsequent implantation.






Reference: fertility.com.hk/
The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.

IVF & ICSI (1)

Infertility treatment :

• IVF (In vitro fertilization), 
• ICSI (Intracytoplasmic sperm injection)

It involves ovarian stimulation to produce a number of eggs, which are collected under ultrasound guidance. With IVF, the eggs are mixed with prepared sperm and fertilization takes place over the next few hours. With lCSl, a single selected sperm is injected into the egg to fertilize it.

The resulting fertilised eggs are allowed to develop for 23 days Embryos are selected for transfer in to the uterus. Following embryo transfer, any remaining suitable embryos are cryopreserved for future transfer.

Indications for IVF may include:

Blocked, damaged or absent fallopian tubes;

Endometriosis;

Ovulatory problem;

Unexplained infertility; and

Sperm problems

ICSI may be applicable in particular when:

The sperm count is very low;

The sperm have low motility;

Very few normal sperm are present;

An attempt at IVF has resulted in failure of fertilization; and

Sperm are retrieved directly from the testis or epididymis using a

surgical technique.

Reference: fertility.com.hk/

The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.

Treatment procedures for infertility problem - Intracytoplasmic Sperm Injection (ICSI) (Part 2)

What does it involve?

To start, you will have to take fertility medications to induce ovulation during your cycle before the ICSI because this procedure requires multiple eggs to ensure the success of the treatment.

 

Similar to standard IVF procedures, the mature eggs are collected from your ovaries and you may receive general anaesthesia during the procedure. Meanwhile, your partner will be asked to provide a semen sample. The sperms are then evaluated and the healthiest sperm are selected for the ICSI procedure.

 

After the eggs and sperms are collected, the ICSI process will take place in the laboratory in which the “healthiest” sperm is injected directly into the centre of the egg by an embryologist.

 

Eggs are observed for the following day or so and those that are fertilised and developed properly will then be transferred into your uterine cavity.

 

If the procedure has been successful, your doctor will discuss with you and your partner about the number of embryos to be transferred to your uterine cavity. Your doctor will place a speculum inside your vagina, insert a small catheter through your cervix into your uterine cavity, and transfer the embryos through the catheter. The transfer causes minimal discomfort.

 

You will be asked to do a pregnancy blood test at the clinic about 2 weeks after the embryo transfer. It is not recommended to do a home pregnancy test before that time because some of the fertility medications may lead to false results if the test is performed too early.

 

 

 

 

Advantages of ICSI

ICSI is the most commonly used method to overcome male infertility problems. By selecting one single “healthiest” sperm and directly injecting into the egg, it bypasses the problem of penetration, which may not be easily done by less healthy sperm in a natural environment, thus enhancing the chance of pregnancy. Other benefits include:

l   Most effective procedure for male infertility

l   Overcoming sperm quality problems

 

 

Disadvantages of ICSI

l   Possibility of transmitting infertility to offspring;

l   Technically demanding; and

l   More expensive than IVF.

 

 

 

 

Reference: fertility.com.hk

The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.

 

Treatment procedures for infertility problem - Intracytoplasmic Sperm Injection (ICSI) (Part 1)

According to the Hong Kong Council on Human Reproductive Technology, a couple may be classified as "infertile" or "sub-infertile" if pregnancy has not occurred after one year of regular unprotected sexual life. In Hong Kong, an estimated one in six couples has difficulty conceiving due to different reasons.

 

There are many reasons for infertility. Problems affecting male fertility include tubal obstruction or damage, semen abnormality or unexplained infertility while those affecting women include ovulation disorders, endometriosis and unexplained infertility. Age may also be one of the other factors affecting fertility.

 

A Reproductive Technology (RT) procedure means a medical, surgical, obstetric or other procedures assisting or bringing about human reproduction by artificial means. RT treatment may be considered as one of the options to increase the chance of conception. Apart from RT treatment, other methods such as timed intercourse, drug therapy and surgery may be recommended by medical practitioners. You are strongly advised to consult your doctor for the most appropriate and suitable procedures if you intend to go through infertility treatment.

 

 

Intracytoplasmic Sperm Injection (ICSI)

ICSI is a method of gamete micromanipulation where a single sperm is injected into the inner cellular structure of an egg. The resulting embryo will be transferred into woman's womb as in a normal IVF cycle.

 

 

 

 

 

 

 

Reference: fertility.com.hk

The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.

 

Kw: ICSI, Intracytoplasmic Sperm Injection, infertility Problems, couples, test tube baby

Treatment procedures for infertility problem - Intracytoplasmic Sperm Injection (ICSI) (Part 2)

What does it involve?

To start, you will have to take fertility medications to induce ovulation during your cycle before the ICSI because this procedure requires multiple eggs to ensure the success of the treatment.

 

Similar to standard IVF procedures, the mature eggs are collected from your ovaries and you may receive general anaesthesia during the procedure. Meanwhile, your partner will be asked to provide a semen sample. The sperms are then evaluated and the healthiest sperm are selected for the ICSI procedure.

 

After the eggs and sperms are collected, the ICSI process will take place in the laboratory in which the “healthiest” sperm is injected directly into the centre of the egg by an embryologist.

 

Eggs are observed for the following day or so and those that are fertilised and developed properly will then be transferred into your uterine cavity.

 

If the procedure has been successful, your doctor will discuss with you and your partner about the number of embryos to be transferred to your uterine cavity. Your doctor will place a speculum inside your vagina, insert a small catheter through your cervix into your uterine cavity, and transfer the embryos through the catheter. The transfer causes minimal discomfort.

 

You will be asked to do a pregnancy blood test at the clinic about 2 weeks after the embryo transfer. It is not recommended to do a home pregnancy test before that time because some of the fertility medications may lead to false results if the test is performed too early.

 

 

 

Advantages of ICSI

ICSI is the most commonly used method to overcome male infertility problems. By selecting one single “healthiest” sperm and directly injecting into the egg, it bypasses the problem of penetration, which may not be easily done by less healthy sperm in a natural environment, thus enhancing the chance of pregnancy. Other benefits include:

l   Most effective procedure for male infertility

l   Overcoming sperm quality problems

 

Disadvantages of ICSI

l   Possibility of transmitting infertility to offspring;

l   Technically demanding; and

l   More expensive than IVF.

 

 

 

 

Reference: fertility.com.hk

 The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.

Treatment procedures for infertility problem - Intracytoplasmic Sperm Injection (ICSI) (Part 1)

According to the Hong Kong Council on Human Reproductive Technology, a couple may be classified as "infertile" or "sub-infertile" if pregnancy has not occurred after one year of regular unprotected sexual life. In Hong Kong, an estimated one in six couples has difficulty conceiving due to different reasons.

 

There are many reasons for infertility. Problems affecting male fertility include tubal obstruction or damage, semen abnormality or unexplained infertility while those affecting women include ovulation disorders, endometriosis and unexplained infertility. Age may also be one of the other factors affecting fertility.

A Reproductive Technology (RT) procedure means a medical, surgical, obstetric or other procedures assisting or bringing about human reproduction by artificial means. RT treatment may be considered as one of the options to increase the chance of conception. Apart from RT treatment, other methods such as timed intercourse, drug therapy and surgery may be recommended by medical practitioners. You are strongly advised to consult your doctor for the most appropriate and suitable procedures if you intend to go through infertility treatment. (reference: http://www.chrt.org.hk/english/patients/patients_abo.html)

 

 

Intracytoplasmic Sperm Injection (ICSI)

ICSI is a method of gamete micromanipulation where a single sperm is injected into the inner cellular structure of an egg. The resulting embryo will be transferred into woman's womb as in a normal IVF cycle.

The information aims to provide educational purpose only. Anyone reading it should consult obstetricians and gynecologists before considering treatment and should not rely on the information above.